INTERNATIONAL 8th USBİLİM HEALTH, ENGINEERING AND APPLIED SCIENCES CONGRESS
DETERMINATION THE BIOLOGICAL ACTIVITIES OF MUSCARİ COMOSUM (L.) MİLL.
Yayıncı:
Akademik Paylaşım Platformu Publishing House - APP Publications
Muscari comosum (L.) Mill. (Asparagaceae), referred to as 'morbaş' in Turkey, is a perennial bulbous, flowering perennial plant belonging to the genus Muscari Mill. It has a natural distribution in Southern and Central Europe, North Africa and Southwest Asia, particularly in the Mediterranean basins. In addition to its use as a functional food and nutritional supplement, M. comosum (MC) has been traditionally reported to be used to treat various diseases and disorders (such as digestive system disorders, antiinflammatory and diuretic effects). This has inspired the study of the pharmacological activities of different parts of the M. comosum species used as drugs. A substantial body of research has demonstrated the diverse biological properties of the MC species, encompassing antioxidant, antidiabetic, anti- obesity, anticancer, antialzheimer's, antibacterial and immunostimulant effects. The limited number of parts of the MC species used as drugs in the studies encourages both traditional folk medicine and modern pharmacological studies to examine it comprehensively, with an emphasis on newer targets. Therefore, the aim of the present study was to examine in detail the biological activities (in vitro) of the above- and below-ground parts of the MC species, followed by mixing all these parts, and to provide data on the potential of the species by highlighting new markers. In this study, ethanol extracts of above and below ground parts of MC species (seed-MCT, root-MCK, branch-MCD, leaf-MCY, flower-MCÇ and mixed-MCM) were prepared separately and their antioxidant and biological activities were investigated. The total phenolic contents of the prepared extracts were determined using Folin-Ciocalteu reagent equivalent to pyrocatechol and total flavonoid contents were determined using aluminium nitrate method equivalent to quercetin. DPPH (2,2-diphenyl-1- picrylhydrazyl, free radical scavenging method), ABTS (2,2'-azino-bis (3- ethylbenzothiazoline-6-sulphonic acid, cation radical scavenging activity method) and CUPRAC (copper (II) reducing antioxidant capacity) were preferred as antioxidant test methods and BHT (butyl hydroxy toluene) and α-TOC (α-tocopherol) were used as standards. In the enzyme activities of the sample, (%) inhibition efficiency on AChE (acetylcholinesterase), BChE (butyrylcholinesterase), urease, tyrosinase, elastase, collagenase and ACE (Angiotensin Converting Enzyme) enzymes were evaluated. When total phenolic and flavonoid contents were analysed, it was determined that the highest phenolic content belonged to MCY (28,53±0,51 μg PEs/mg) sample and the highest value in terms of flavonoids belonged to MCK (12,28 μg QEs/mg) sample. In ABTS method, MCK (IC50: 20,06±0,19 μg/mL) sample showed the highest activity. This value was slightly higher compared to BHT (17.07±0.11 μg/mL) and α-TOC (8.43±0.10 μg/mL) standards. This shows that the MCK sample shows antioxidant activity but not as effective as the standards. In the CUPRAC method, MCK (A0.5: 81.58±1.01 μg/mL) sample was the most active sample with a high A0.5 value among all samples compared to standards such as BHT (7.99±0.12 μg/mL) and α-TOC (18.07±0.08 μg/mL) and it can be said that it has a significant antioxidant potential. In terms of ACE inhibition, MCK (inhibition%: 99.50±1.05) and MCM (inhibition%: 99.50±1.02) samples exhibited almost equivalent inhibition activity against standard lisinopril (inhibition%: 99.98±1.36). The antioxidant and biological activities of MC species were investigated and the results were presented to the scientific world. As a result, different parts of the MC species, especially the MCK sample, exhibited a remarkable biological activity in terms of antioxidant and certain enzyme inhibition activities. While it shows moderate activity in terms of antioxidant activities compared to standard compounds, the fact that it shows similar inhibition activity with lisinopril in terms of enzyme inhibition, especially in ACE inhibition, supports the pharmaceutical potential of MCK sample, it is thought that it may have antihypertensive effects and can be evaluated as a natural source in the treatment of hypertension.
