EGE 12TH INTERNATIONAL CONFERENCE ON APPLIED SCIENCES
EVALUATION OF THE EFFECT OF CURCUMIN AND ELLAGIC ACID ON LUCIDIN-INDUCED DNA DAMAGE TO HUMAN PERIPHERAL LYMPHOCYTES BY COMET ASSAY
Yayıncı:
Academy Global Publishing House
Lucidin is an anthraquinone derivative metabolite obtained from the root dye plant and used as a dyestuff. Curcumin and ellagic acid are polyphenol-group antioxidants that have recently gained popularity. In this study, the antigenotoxic effects of ellagic acid and curcumin against the possible genotoxicity of lucidin in human peripheral blood lymphocytes were investigated with the comet assay, known as a single-cell gel electrophoresis test method. The study included a negative control (10% DMSO), positive control (EMS; 1 mM), lucidin (LUC; 200 μM), ellagic acid (EA; 50 and 100 μM), curcumin (CUR; 0.1 and 1 μM), LUC+EA, LUC+CUR, and LUC+EA+CUR groups. Counting cells under a fluorescent microscope provided the mean percentage of damaged cells, genetic damage index, tail length, tail DNA density (%), and tail moment. Using the SPSS package program, the results were compared to the negative control group. Genetic damage index, percentage of damaged cells, tail length, and tail moment parameters increased significantly in the LUC group compared to the control. While these values were lower in all EA and CUR groups compared to the control, it was observed that they approached the control in the LUC+EA, LUC+CUR, and LUC+EA+CUR combination groups. As a result, it was observed that LUC induces DNA damage in human peripheral blood lymphocytes, exhibiting a toxic effect. However, the combination of EA and CUR reduced LUC toxicity through their antigenotoxic effects. The study aimed to assess the DNA-protective capabilities of EA and CUR.
