THE INTERNATIONAL CONGRESS ON INFORMATION TECHNOLOGIES IN MEDICINE, PHARMACY, AGRICULTURE, FOOD, FORESTY, ENVIRONMENT AND ENGINEERING
Importance of Rapid Perfusion System in Characterizing Acid-Sensing Ion Channels (ASICs) in Mouse Brain Tissue: An Electrophysiological Study
Yazarlar:
Ziya Cakir
Kübra Cayli
Bedrettin Cinpolat
Yayıncı:
Tokat Gaziosmanpaşa Üniversitesi
Acid-sensitive ion channels (ASIC) are a subgroup of the proton-gated voltage-independent degenerin/epithelial sodium channel (DEG/ENaC) superfamily. These channels are ion channels sensitive to hydrogen ions (acid). They are commonly found in both the central nervous system and the peripheral nervous system. There are six known subgroups of ASICs, namely ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3, and ASIC4. In electrophysiological studies, a normal perfusion system is used and the fluid is continuously perfused at a rate of 4-5 ml/minute. However, ASIC channels are ion channels that are rapidly activated and desensitized. Therefore, we designed a rapid perfusion system to record from ASIC channels in electrophysiological studies. The rapid perfusion system continuously perfused the fluid at a speed of 15 ml/minute. We recorded ASIC currents using the rapid perfusion system because these channels activate and desensitize quickly. Additionally, during recording, the entry of the perfusion solution should be aligned with the recorded cell on the cross-section. Since acid-sensing ion channels are rapidly desensitizing channels, at least 6 minutes should be waited to re-induce the activation of ASIC currents. Because after applying the acidic solution, currents did not occur in applications of acidic solutions less than 6minutes. In this study, a rapid perfusion system was used to characterize the electrophysiological properties of ASICs in brain tissue. It is believed that our study is unique because it is the first time ASIC channels in brain tissue neurons are being studied using a rapid perfusion system. Our study presents, for the first time, the characterization of the biophysical and pharmacological properties of ASIC currents in brain slices. Therefore, in this study, the presence of ASIC currents in brain slices was investigated using the patch clamp electrophysiological approach with rapid perfusion, and the biophysical and pharmacological properties of these channels were characterized and contributed to the literature.
