MEDICAL INFORMATICS IV
VIRTUAL CROSSMATCHING AND EPITOPE MAPPING FOR BETTER DONOR MATCH IN KIDNEY TRANSPLANTATION
Yazarlar:
Sedat Tanju KARADENİZ
E. Demet AKTEN
S. Rüştü OĞUZ
Çiğdem KEKLİK ÇINAR
Hayriye ŞENTÜRK ÇİFTÇİ
Fatma SAVRAN OĞUZ
Kemal YELEKCİ
Yayıncı:
İstanbul Üniversitesi Yayınları
The human leukocyte antigen (HLA) disparity between donors and recipients is the primary driver of donor specific antibodies (DSA) formation and graft rejection after transplantation. It was aimed to predict the DSA by finding the HLA antigen mismatches, searching the eplets of antigens that bind to the recipient’s anti-HLA antibodies, calculating the number of shared eplets between the mismatched donor HLA antigens and the recipient’s pretransplantation anti-HLA antibody-bound antigens. Recipient-donor HLA Typing results and the recipient’s pretransplantation and post-transplantation anti-HLA antibody detection results by Luminex single antigen bead (Luminex-SAB) assay were used as retrospective data for calculation in five steps. In the first step, HLA Typing results were compared to find the mismatched and in the second step the relevant eplets were searched for the recipient’s pre-transplantation anti-HLA antibodies. Next, the shared eplets between the donor’s mismatched HLA antigens and the recipient’s pre-transplantation anti-HLA antibodies were calculated to find the highest number of shares. In the fourth step, the most shared anti-HLA antibodies were listed as the most probable DSA. Finally, the possible epitope’s peptide AA (amino acid) sequences were confirmed with the “IEDB Bepipred-1.0 Antibody Epitope Prediction method” using the donor’s HLA antigen AA sequence.
