CURRENT TRENDS IN NEUROSCIENCES
NEURONAL CULTURE METHODS TO STUDY NEURONAL FUNCTION AND DISEASE
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İstanbul Üniversitesi Yayınları
Damage to the nervous system often triggers irreversible degenerative process. Trauma to the central nervous system in particular causes severe loss of function. The most important reason for this is that neurons do not divide. The inability to replace the deceased and the disruption of axonal connections regeneration is not restored by regeneration. Therefore, there are still many incurables develop treatment strategies for neurological disease or nervous system trauma. Many researchers aiming to understand neurodegenerative and neurodegenerative processes are currently working on these diseases for which there is no definitive cure. Primary in vitro cultures of neurons have been widely used in recent years to improve our understanding of the functioning of the nervous system. Primary neuronal cells are particularly difficult to purify and culture because neurons do not undergo cell division and are surrounded by large numbers of glia cells to minimize both time and cost, immortalized secondary cell lines derived from neuronal tumors have been tried. They have the advantage of providing unlimited cell numbers and minimizing variability between cultures, as well as being relatively easy to grow in cell culture. The major disadvantage of these cell lines is that they have many important physiological differences with the cell type from which they are derived and are not very amenable to manipulation. Often such cell lines are induced to exhibit a more neuronal phenotype by changing culture conditions, adding factors, etc. In this section, the main characteristics and common uses of neuronal cultures will be discussed
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