6. INTERNATIONAL CONGRESS ON CONTEMPORARY SCIENTIFIC RESEARCH
SERTRALINE SUPPRESSES THE PROLIFERATION OF CACO-2 CELLS BY INDUCING FERROPTOSIS.
Colorectal cancer (CRC) is the second most lethal cancer and the third most common malignancy worldwide. Surgery, chemotherapy, and targeted therapy have been widely used to treat CRC, but some patients still develop resistance to these treatments. Ferroptosis is a novel non-apoptotic form of cell death. It is an iron-dependent nonapoptotic cell death characterized by the accumulation of lipid reactive oxygen species and has been suggested to play a role in reversing resistance to anticancer drugs. The aim of this study was to investigate the effect of sertraline on Caco-2 cells via ferroptosis. We evaluated the effects of sertraline on cell viability at various concentrations (0–50 μM) using MTT assay. ACSL4 and GPx4 levels were evaluated in Caco-2 cells subjected to Sertraline treatment. Sertraline’s low and IC50 concentrations were determined as 5 and 15 μM, respectively. Proliferation in cells treated with 5, 15 and 40 μM sertraline concentrations for 24 h was decreased dose-dependently at 15 and 40 μM. Sertraline increased ACSL4 levels and decreased GPx4 levels in a concentration-dependent manner. The effect of sertraline in the treatment of colorectal cancer via ferroptosis should be supported by clinical studies.
