II. EURASIA INTERNATIONAL CONGRESS OF HEALTH SCIENCES
CHEMICAL COMPOSITION OF PEDICULARIS SIBTHORPII VAR. ERIANTHA AND ANTIOXIDANT ACTIVITY
Yayıncı:
Liberty Publications
Plants have historically served as a cornerstone for discovering bioactive compounds with therapeutic properties. Among these, phenolic compounds are particularly noted for their antioxidant, antiinflammatory, and antimicrobial activities. The Pedicularis genus, comprising over 600 species within the Orobanchaceae family, has garnered significant interest due to its traditional medicinal uses and rich phytochemical profile. The Nakhchivan Autonomous Republic, known for its rich biodiversity and diverse climatic conditions, offers a favorable environment for the growth of Pedicularis genus plants. Six species of this genus have been identified across the region's various ecosystems. Predominantly found in subalpine and alpine meadows, these plants are notable for their ability to thrive under challenging ecological conditions, such as high temperatures and low oxygen levels. Pedicularis sibthorpii var. eriantha was collected from the Batabat area of Nakhchivan (2251m). This article explores the chemical composition and antioxidant activities of Pedicularis sibthorpii var. eriantha, as well as its numerous purported therapeutic properties. This study investigates the chemical composition and antioxidant potential of Pedicularis sibthorpii var. eriantha, a plant collected from the Batabat area of Nakhchivan (2251 m). LC-MS/MS analysis revealed a phytochemical profile rich in flavonoids and phenolic acids, with kaempferol (0.049 mg/g extract), isoquercitrin (0.033 mg/g extract), and rutin (0.021 mg/g extract) being the most abundant compounds. These flavonoids significantly contribute to the plant's potent antioxidant and anti-inflammatory properties. Phenolic acids, including syringic acid (0.019 mg/g extract), trans-cinnamic acid (0.015 mg/g extract), and vanillic acid (0.010 mg/g extract), also demonstrated strong free radical scavenging and anti-inflammatory activities. The antioxidant potential of the extract was evaluated using the DPPH, Folin-Ciocalteu Reagent (FCR), and FRAP assays, employing the novel biosensor method. The FCR assay revealed a high phenolic content of 491.79 mg GA/g, which surpassed the levels found in standard antioxidants such as Vitamin C (452.81 mg GA/g) and Trolox (296.86 mg GA/g). In the FRAP assay, the extract demonstrated moderate reducing capacity (16.55 µmol Trolox/g), slightly lower than Trolox (18.73 µmol Trolox/g) and Vitamin C (22.45 µmol Trolox/g). The DPPH assay indicated strong free radical scavenging activity at 86.62%, comparable to powerful antioxidants such as Vitamin C (82.35%) and Gallic acid (90.63%). These findings suggest that P. sibthorpii var. eriantha is a promising natural source of antioxidants, with potential applications in combating oxidative stress and related diseases. This study establishes a scientific foundation for the traditional medicinal use of P. Sibthorpii var. eriantha and underscores its potential for pharmacological applications. The high concentrations of kaempferol and other bioactive compounds suggest its utility in developing natural antioxidants and therapeutic agents. By advancing the understanding of P. Sibthorpii var. eriantha, this research contributes to the broader exploration of alpine medicinal plants and their role in combating oxidative stress and related diseases.
